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human hgsoc cell line ov90  (ATCC)


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    ATCC human hgsoc cell line ov90
    (a) HIF1a western blot analysis in representative OV-90 +/+ and <t>OV90</t> −/− xenografts (n=3 per group). Calreticulin was used as loading control. (b) Gene expression of HIF1 targets evaluated by qRT-PCR in OV-90 +/+ (n=5) and OV-90 −/− (n=6) xenografts. Data were log transformed prior to statistical analysis. (c) Masson’s trichrome staining of OV-90 +/+ and OV90 −/− xenografts, with respective quantification of the stroma (collagen areas stained in blue). Representative images are shown with black squares indicating the insets of the areas shown at higher magnification. (d) Immunofluorescent staining of endomucin (Endo) and Smooth Muscle Actin (SMA) in OV-90 +/+ and OV-90 −/− xenografts, with nuclei stained with DAPI, scale bar 200 µm. White squares indicate the insets of the areas shown at higher magnification. Representative images are shown, together with quantification of total (Endo + ) and pericyte negative (Endo + SMA − ) vessels. (e) Ultrasound images of OV-90 +/+ and OV-90 −/− xenografts. Incoming (red) and outcoming (blue) blood flows are shown. The graph indicates the percentage of the blood flow areas detected within each tumor (n=3 per group). One-tailed t-test was used to compare averages between the groups. (f) Immunofluorescent staining of F4/80 + macrophages (red) in OV-90 +/+ and OV-90 −/− xenografts. White squares indicate the insets of the areas shown at higher magnification. The arrows indicate F4/80 + cells. Nuclei are stained with DAPI (blue), scale bar = 20 µm. For all panels, data in graphs are represented as mean ± SEM and p-value is indicated.
    Human Hgsoc Cell Line Ov90, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hgsoc+cell+line+ov90/OV-90/bio_rxiv__64898__2026__02__28__708681-76-1-9
    Average 96 stars, based on 421 article reviews
    human hgsoc cell line ov90 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy"

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy

    Journal: bioRxiv

    doi: 10.64898/2026.02.28.708681

    (a) HIF1a western blot analysis in representative OV-90 +/+ and OV90 −/− xenografts (n=3 per group). Calreticulin was used as loading control. (b) Gene expression of HIF1 targets evaluated by qRT-PCR in OV-90 +/+ (n=5) and OV-90 −/− (n=6) xenografts. Data were log transformed prior to statistical analysis. (c) Masson’s trichrome staining of OV-90 +/+ and OV90 −/− xenografts, with respective quantification of the stroma (collagen areas stained in blue). Representative images are shown with black squares indicating the insets of the areas shown at higher magnification. (d) Immunofluorescent staining of endomucin (Endo) and Smooth Muscle Actin (SMA) in OV-90 +/+ and OV-90 −/− xenografts, with nuclei stained with DAPI, scale bar 200 µm. White squares indicate the insets of the areas shown at higher magnification. Representative images are shown, together with quantification of total (Endo + ) and pericyte negative (Endo + SMA − ) vessels. (e) Ultrasound images of OV-90 +/+ and OV-90 −/− xenografts. Incoming (red) and outcoming (blue) blood flows are shown. The graph indicates the percentage of the blood flow areas detected within each tumor (n=3 per group). One-tailed t-test was used to compare averages between the groups. (f) Immunofluorescent staining of F4/80 + macrophages (red) in OV-90 +/+ and OV-90 −/− xenografts. White squares indicate the insets of the areas shown at higher magnification. The arrows indicate F4/80 + cells. Nuclei are stained with DAPI (blue), scale bar = 20 µm. For all panels, data in graphs are represented as mean ± SEM and p-value is indicated.
    Figure Legend Snippet: (a) HIF1a western blot analysis in representative OV-90 +/+ and OV90 −/− xenografts (n=3 per group). Calreticulin was used as loading control. (b) Gene expression of HIF1 targets evaluated by qRT-PCR in OV-90 +/+ (n=5) and OV-90 −/− (n=6) xenografts. Data were log transformed prior to statistical analysis. (c) Masson’s trichrome staining of OV-90 +/+ and OV90 −/− xenografts, with respective quantification of the stroma (collagen areas stained in blue). Representative images are shown with black squares indicating the insets of the areas shown at higher magnification. (d) Immunofluorescent staining of endomucin (Endo) and Smooth Muscle Actin (SMA) in OV-90 +/+ and OV-90 −/− xenografts, with nuclei stained with DAPI, scale bar 200 µm. White squares indicate the insets of the areas shown at higher magnification. Representative images are shown, together with quantification of total (Endo + ) and pericyte negative (Endo + SMA − ) vessels. (e) Ultrasound images of OV-90 +/+ and OV-90 −/− xenografts. Incoming (red) and outcoming (blue) blood flows are shown. The graph indicates the percentage of the blood flow areas detected within each tumor (n=3 per group). One-tailed t-test was used to compare averages between the groups. (f) Immunofluorescent staining of F4/80 + macrophages (red) in OV-90 +/+ and OV-90 −/− xenografts. White squares indicate the insets of the areas shown at higher magnification. The arrows indicate F4/80 + cells. Nuclei are stained with DAPI (blue), scale bar = 20 µm. For all panels, data in graphs are represented as mean ± SEM and p-value is indicated.

    Techniques Used: Western Blot, Control, Gene Expression, Quantitative RT-PCR, Transformation Assay, Staining, One-tailed Test

    Related Articles

    Western Blot:

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy
    Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).. Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.

    Control:

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy
    Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).. Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.

    Gene Expression:

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy
    Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).. Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.

    Quantitative RT-PCR:

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy
    Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).. Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.

    Transformation Assay:

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy
    Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).. Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.

    Staining:

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy
    Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).. Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.

    One-tailed Test:

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy
    Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).. Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.Cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) high glucose (Euroclone #ECM0749L), supplemented with 10% FBS (Euroclone #ECS0180L), L-glutamine (2 mM, Euroclone #ECB3000D), penicillin/streptomycin (1×, Euroclone #ECB3001D), uridine (50 μg/mL, Sigma-Aldrich #U3003), in an incubator with a humidified atmosphere at 5% CO2 and 37 °C.



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    human hgsoc cell line ov90  (ATCC)
    96
    ATCC human hgsoc cell line ov90
    (a) HIF1a western blot analysis in representative OV-90 +/+ and <t>OV90</t> −/− xenografts (n=3 per group). Calreticulin was used as loading control. (b) Gene expression of HIF1 targets evaluated by qRT-PCR in OV-90 +/+ (n=5) and OV-90 −/− (n=6) xenografts. Data were log transformed prior to statistical analysis. (c) Masson’s trichrome staining of OV-90 +/+ and OV90 −/− xenografts, with respective quantification of the stroma (collagen areas stained in blue). Representative images are shown with black squares indicating the insets of the areas shown at higher magnification. (d) Immunofluorescent staining of endomucin (Endo) and Smooth Muscle Actin (SMA) in OV-90 +/+ and OV-90 −/− xenografts, with nuclei stained with DAPI, scale bar 200 µm. White squares indicate the insets of the areas shown at higher magnification. Representative images are shown, together with quantification of total (Endo + ) and pericyte negative (Endo + SMA − ) vessels. (e) Ultrasound images of OV-90 +/+ and OV-90 −/− xenografts. Incoming (red) and outcoming (blue) blood flows are shown. The graph indicates the percentage of the blood flow areas detected within each tumor (n=3 per group). One-tailed t-test was used to compare averages between the groups. (f) Immunofluorescent staining of F4/80 + macrophages (red) in OV-90 +/+ and OV-90 −/− xenografts. White squares indicate the insets of the areas shown at higher magnification. The arrows indicate F4/80 + cells. Nuclei are stained with DAPI (blue), scale bar = 20 µm. For all panels, data in graphs are represented as mean ± SEM and p-value is indicated.
    Human Hgsoc Cell Line Ov90, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hgsoc+cell+line+ov90/OV-90/bio_rxiv__64898__2026__02__28__708681-76-1-9
    Average 96 stars, based on 1 article reviews
    human hgsoc cell line ov90 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    human hgsoc cell lines ov90  (ATCC)
    96
    ATCC human hgsoc cell lines ov90
    Fig. 1 Co-culture with <t>HGSOC</t> cell lines promoted the differentiation and polarization of primary human macrophages towards the alternatively activated macrophage (AAM) phenotype. a Immunofluorescent detection of CD68 (a macrophage marker, green) and CD163 (an AAM marker, green) in monocytes that have been co-cultured with HGSOC cell lines for 7 days. Cells were counterstained with DAPI (blue). Scale bar = 250 μm. b-e Percentage of cells that were CD68+ (b), number of CD68+ cells per field of view (C), percentage of cells that were CD163+ (d), and number of CD163+ cells per field of view (e) after monocytes have been co-cultured with HGSOC cell lines for 7 days. Data are expressed as average ± SD, n = 3 monocyte donors. Groups with no statistically significant differences are linked by the same letters, while groups that are statistically different from each other have different letters, p < 0.05 by Tukey-HSD
    Human Hgsoc Cell Lines Ov90, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hgsoc+cell+line+ov90/OV-90/pm33069212-52-0-10
    Average 96 stars, based on 1 article reviews
    human hgsoc cell lines ov90 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) HIF1a western blot analysis in representative OV-90 +/+ and OV90 −/− xenografts (n=3 per group). Calreticulin was used as loading control. (b) Gene expression of HIF1 targets evaluated by qRT-PCR in OV-90 +/+ (n=5) and OV-90 −/− (n=6) xenografts. Data were log transformed prior to statistical analysis. (c) Masson’s trichrome staining of OV-90 +/+ and OV90 −/− xenografts, with respective quantification of the stroma (collagen areas stained in blue). Representative images are shown with black squares indicating the insets of the areas shown at higher magnification. (d) Immunofluorescent staining of endomucin (Endo) and Smooth Muscle Actin (SMA) in OV-90 +/+ and OV-90 −/− xenografts, with nuclei stained with DAPI, scale bar 200 µm. White squares indicate the insets of the areas shown at higher magnification. Representative images are shown, together with quantification of total (Endo + ) and pericyte negative (Endo + SMA − ) vessels. (e) Ultrasound images of OV-90 +/+ and OV-90 −/− xenografts. Incoming (red) and outcoming (blue) blood flows are shown. The graph indicates the percentage of the blood flow areas detected within each tumor (n=3 per group). One-tailed t-test was used to compare averages between the groups. (f) Immunofluorescent staining of F4/80 + macrophages (red) in OV-90 +/+ and OV-90 −/− xenografts. White squares indicate the insets of the areas shown at higher magnification. The arrows indicate F4/80 + cells. Nuclei are stained with DAPI (blue), scale bar = 20 µm. For all panels, data in graphs are represented as mean ± SEM and p-value is indicated.

    Journal: bioRxiv

    Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy

    doi: 10.64898/2026.02.28.708681

    Figure Lengend Snippet: (a) HIF1a western blot analysis in representative OV-90 +/+ and OV90 −/− xenografts (n=3 per group). Calreticulin was used as loading control. (b) Gene expression of HIF1 targets evaluated by qRT-PCR in OV-90 +/+ (n=5) and OV-90 −/− (n=6) xenografts. Data were log transformed prior to statistical analysis. (c) Masson’s trichrome staining of OV-90 +/+ and OV90 −/− xenografts, with respective quantification of the stroma (collagen areas stained in blue). Representative images are shown with black squares indicating the insets of the areas shown at higher magnification. (d) Immunofluorescent staining of endomucin (Endo) and Smooth Muscle Actin (SMA) in OV-90 +/+ and OV-90 −/− xenografts, with nuclei stained with DAPI, scale bar 200 µm. White squares indicate the insets of the areas shown at higher magnification. Representative images are shown, together with quantification of total (Endo + ) and pericyte negative (Endo + SMA − ) vessels. (e) Ultrasound images of OV-90 +/+ and OV-90 −/− xenografts. Incoming (red) and outcoming (blue) blood flows are shown. The graph indicates the percentage of the blood flow areas detected within each tumor (n=3 per group). One-tailed t-test was used to compare averages between the groups. (f) Immunofluorescent staining of F4/80 + macrophages (red) in OV-90 +/+ and OV-90 −/− xenografts. White squares indicate the insets of the areas shown at higher magnification. The arrows indicate F4/80 + cells. Nuclei are stained with DAPI (blue), scale bar = 20 µm. For all panels, data in graphs are represented as mean ± SEM and p-value is indicated.

    Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).

    Techniques: Western Blot, Control, Gene Expression, Quantitative RT-PCR, Transformation Assay, Staining, One-tailed Test

    Fig. 1 Co-culture with HGSOC cell lines promoted the differentiation and polarization of primary human macrophages towards the alternatively activated macrophage (AAM) phenotype. a Immunofluorescent detection of CD68 (a macrophage marker, green) and CD163 (an AAM marker, green) in monocytes that have been co-cultured with HGSOC cell lines for 7 days. Cells were counterstained with DAPI (blue). Scale bar = 250 μm. b-e Percentage of cells that were CD68+ (b), number of CD68+ cells per field of view (C), percentage of cells that were CD163+ (d), and number of CD163+ cells per field of view (e) after monocytes have been co-cultured with HGSOC cell lines for 7 days. Data are expressed as average ± SD, n = 3 monocyte donors. Groups with no statistically significant differences are linked by the same letters, while groups that are statistically different from each other have different letters, p < 0.05 by Tukey-HSD

    Journal: BMC cancer

    Article Title: Ovarian cancer cells direct monocyte differentiation through a non-canonical pathway.

    doi: 10.1186/s12885-020-07513-w

    Figure Lengend Snippet: Fig. 1 Co-culture with HGSOC cell lines promoted the differentiation and polarization of primary human macrophages towards the alternatively activated macrophage (AAM) phenotype. a Immunofluorescent detection of CD68 (a macrophage marker, green) and CD163 (an AAM marker, green) in monocytes that have been co-cultured with HGSOC cell lines for 7 days. Cells were counterstained with DAPI (blue). Scale bar = 250 μm. b-e Percentage of cells that were CD68+ (b), number of CD68+ cells per field of view (C), percentage of cells that were CD163+ (d), and number of CD163+ cells per field of view (e) after monocytes have been co-cultured with HGSOC cell lines for 7 days. Data are expressed as average ± SD, n = 3 monocyte donors. Groups with no statistically significant differences are linked by the same letters, while groups that are statistically different from each other have different letters, p < 0.05 by Tukey-HSD

    Article Snippet: Human HGSOC cell lines OV90 and OVCAR3 were purchased from ATCC (Manassas, Virginia), OVCA432 and OVCAR5 were obtained from Dr. R. Bast (MD Anderson Cancer Center; Houston, TX, USA), and OVCAR4 were obtained from the NCI Tumor Repository (Frederick, MD).

    Techniques: Co-Culture Assay, Marker, Cell Culture

    Fig. 2 The pro-AAM potential of the HGSOC cell lines was not correlated with the secretion of established pro-AAM factors. a Gene expression (Z-score normalized) from HGSOC cell lines in co-culture compared to their pro-AAM potential (the resulting number of CD68+ and CD163+ cells after 7 days of co-culture). Values represent the average of 4 microdevices. b CD68+ per field of view and (c) 163+ cells per field of view after monocytes have been alternatively activated using MCSF, IL4, and IL13 (AAMs) or co-cultured with HGSOC cell lines OVCAR5 and OVCA432 for 7 days, +/−an anti-MCSF antibody. Data are expressed as average ± SD, n = 4 microdevices per condition; *p < 0.05 vs. Veh. by two-way ANOVA with Tukey multiple comparison correction. d Cytokine expression (Z-score normalized) from HGSOC cell lines in co-culture compared to their pro-AAM potential. Values represent the average of 4 microdevices

    Journal: BMC cancer

    Article Title: Ovarian cancer cells direct monocyte differentiation through a non-canonical pathway.

    doi: 10.1186/s12885-020-07513-w

    Figure Lengend Snippet: Fig. 2 The pro-AAM potential of the HGSOC cell lines was not correlated with the secretion of established pro-AAM factors. a Gene expression (Z-score normalized) from HGSOC cell lines in co-culture compared to their pro-AAM potential (the resulting number of CD68+ and CD163+ cells after 7 days of co-culture). Values represent the average of 4 microdevices. b CD68+ per field of view and (c) 163+ cells per field of view after monocytes have been alternatively activated using MCSF, IL4, and IL13 (AAMs) or co-cultured with HGSOC cell lines OVCAR5 and OVCA432 for 7 days, +/−an anti-MCSF antibody. Data are expressed as average ± SD, n = 4 microdevices per condition; *p < 0.05 vs. Veh. by two-way ANOVA with Tukey multiple comparison correction. d Cytokine expression (Z-score normalized) from HGSOC cell lines in co-culture compared to their pro-AAM potential. Values represent the average of 4 microdevices

    Article Snippet: Human HGSOC cell lines OV90 and OVCAR3 were purchased from ATCC (Manassas, Virginia), OVCA432 and OVCAR5 were obtained from Dr. R. Bast (MD Anderson Cancer Center; Houston, TX, USA), and OVCAR4 were obtained from the NCI Tumor Repository (Frederick, MD).

    Techniques: Gene Expression, Co-Culture Assay, Cell Culture, Comparison, Expressing

    Fig. 3 RNA-seq analysis of five HGSOC cell lines revealed global transcriptional heterogeneity. a Pearson correlations of gene expression in the HGSOC cell lines OV90, OVCAR3, OVCAR4, OVCAR5, and OVCA432 organized using hierarchal clustering along both rows and columns (n = 4). Scale bar represents coefficient of correlation (r). b Principal component analysis of variance stabilized expression data based on weighted gene lists. X and Y-axes represent the primary and secondary principal components (PC1, PC2), respectively, and report overall contribution to variance in the dataset. Extension to a third PC captured an additional 17% variance but did not improve interpretation of the data. While PC2 separated the two cell lines with the strongest macrophage-inducing potential (OVCAR5 and OVCA432) from the other lines, this analysis did not identify potential candidates due to the sheer number of genes that had positive loadings in PC2. c Number of genes found to be differentially expressed (two-fold up- or down-regulated) in the OVCAR3, OVCAR4, OVCAR5, and OVCA432 cell lines relative to OV90

    Journal: BMC cancer

    Article Title: Ovarian cancer cells direct monocyte differentiation through a non-canonical pathway.

    doi: 10.1186/s12885-020-07513-w

    Figure Lengend Snippet: Fig. 3 RNA-seq analysis of five HGSOC cell lines revealed global transcriptional heterogeneity. a Pearson correlations of gene expression in the HGSOC cell lines OV90, OVCAR3, OVCAR4, OVCAR5, and OVCA432 organized using hierarchal clustering along both rows and columns (n = 4). Scale bar represents coefficient of correlation (r). b Principal component analysis of variance stabilized expression data based on weighted gene lists. X and Y-axes represent the primary and secondary principal components (PC1, PC2), respectively, and report overall contribution to variance in the dataset. Extension to a third PC captured an additional 17% variance but did not improve interpretation of the data. While PC2 separated the two cell lines with the strongest macrophage-inducing potential (OVCAR5 and OVCA432) from the other lines, this analysis did not identify potential candidates due to the sheer number of genes that had positive loadings in PC2. c Number of genes found to be differentially expressed (two-fold up- or down-regulated) in the OVCAR3, OVCAR4, OVCAR5, and OVCA432 cell lines relative to OV90

    Article Snippet: Human HGSOC cell lines OV90 and OVCAR3 were purchased from ATCC (Manassas, Virginia), OVCA432 and OVCAR5 were obtained from Dr. R. Bast (MD Anderson Cancer Center; Houston, TX, USA), and OVCAR4 were obtained from the NCI Tumor Repository (Frederick, MD).

    Techniques: RNA Sequencing, Gene Expression, Expressing

    Fig. 4 RNA-seq analyzed by EBSeq identified genes whose expression mirrored the pro-AAM potential of the HGSOC cell lines. a Diagram of the process by which the nine factors of interest were identified. b Gene expression for the nine factors (Z-score normalized) in HGSOC cell lines compared to their pro-AAM potential; values represent the average of 4 microdevices

    Journal: BMC cancer

    Article Title: Ovarian cancer cells direct monocyte differentiation through a non-canonical pathway.

    doi: 10.1186/s12885-020-07513-w

    Figure Lengend Snippet: Fig. 4 RNA-seq analyzed by EBSeq identified genes whose expression mirrored the pro-AAM potential of the HGSOC cell lines. a Diagram of the process by which the nine factors of interest were identified. b Gene expression for the nine factors (Z-score normalized) in HGSOC cell lines compared to their pro-AAM potential; values represent the average of 4 microdevices

    Article Snippet: Human HGSOC cell lines OV90 and OVCAR3 were purchased from ATCC (Manassas, Virginia), OVCA432 and OVCAR5 were obtained from Dr. R. Bast (MD Anderson Cancer Center; Houston, TX, USA), and OVCAR4 were obtained from the NCI Tumor Repository (Frederick, MD).

    Techniques: RNA Sequencing, Expressing, Gene Expression